Technical Data
A2298-74B3
Atg3 (Autophagy-related Protein 3, APG3, APG3-like, AUT1, Protein PC3-96)
Description:
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. ATG3 is an E2-like protein-conjugating enzyme for GABARAPL1, GABARAPL2 and MAPLC3 binding them to PE. ATG3 forms an E1-E2 complex with ATG7 and cross-talks with the ATG12 conjugation system. ATG3 is a cytoplasmic protein that is widely expressed with highest expression in heart, skeletal muscle, kidney, liver and placenta.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20°C. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9G311Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Partial recombinant human ATG3 (1-100) with a GST tag.
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
Specificity:
Recognizes human ATG3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.