Technical Data
A2298-74C
APE (Ref-1, Apurinic Apyrimidinic Endonuclease, APEX, APE-1, HAP1)
Description:
Reactive oxygen species (H2O2, OH, O2, HOCL) generated during normal cellular activities or by ionizing radiation and by many oxidizing/alkylating agents can induce a wide variety of lesions in DNA ranging from oxidized bases (8-oxoguanine and thymine glycol), base loss and DNA strand breaks with modified ends such as 3'-phosphate and 3'-phopshoglycolate. Unrepaired damaged/modified DNA has been implicated in aging and cancer. Many proteins that are involved in BER (base excision repair) have been identified in E Coli and mammals. Repair of modified is initiated by their removal by specific DNA glycosylase resulting in AP (apurinic/apyrimidinic) sites. A series of steps starting with the cleavage of the DNA strand adjacent to AP sites, removal of the deoxyribose phosphate residue, followed by gap filling synthesis and ligation. The DNA strand cleavage is catalyzed by a variety of AP-endonucleases (APE or HAP1, APEX, APE-1) that cleave 5' to the AP sites. APE also has an associated 3'-endonuclease activity the removes 3'trans-a,b-unsaturated aldehyde generated by AP lyase, as well as 3'-phosphate and 3'-phosphoglycolate. ROS generated 3'-blocked termini at the site of strand breaks are also removed APE.

Applications:
Suitable for use in ELISA, Western Blotting. Other applications not tested.

Recommended Dilution:
Western Blot: An initial dilution of 1:1K-2K is recommended for Western. The antibody has detected ~34kD band from human cells and tissues.
ELISA: 1:10,000-50,000 using 50-100ng control peptide/well.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceMouseRabbit
Concentration:
As reported
Immunogen:
Full length mouse APE expressed in E. coli and purified (36kD).
Purity:
Serum
Form
Supplied as a liquid, neat serum, 40% glycerol.
Specificity:
Reacts with mouse and rat but poorly with human APE. Mouse and human APE genes encodes a protein of 318aa. There is 94% sequence identity between human and mouse APE.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Seki S et al (1992) BBA 1131, 287-299; Akiyama K et al (1994) BBA 1219, 15-25; Izumi T et al (1996) Biochem. 35, 14679-14683; Wilson DM & Thompson LH (1997) PNAS 94, 12754-1275