Technical Data
A2298-74F
APE (Ref-1, Apurinic Apyrimidinic Endonuclease)
Description:
Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.2-1.0ug/ml; 35kD band seen in A431 lysate.
Optimal dilutions to be determined by the researcher.

Positive Control:
A431 lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Peptide with sequence PKRGKKGAVAEDGD, from N-Terminus of the protein sequence according to NP_001632; NP_542379; NP_542380.
Purity:
Purified from goat serum by ammonium sulfate precipitation followed by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes APE. Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
General:
Demple B, Herman T, Chen DS.Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes.Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11450-4. PMID: 1722334