Suitable for use in Western Blot. Other applications not tested.
Western Blot: 0.2-1.0ug/ml; –35kD band seen in A431 lysate.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Peptide with sequence PKRGKKGAVAEDGD, from N-Terminus of the protein sequence according to NP_001632; NP_542379; NP_542380. |
|Purified from goat serum by ammonium sulfate precipitation followed by immunoaffinity chromatography.|
|Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.|
|Recognizes APE. Species Crossreactivity: Human.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
Demple B, Herman T, Chen DS.Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes.Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11450-4. PMID: 1722334