Technical Data
A2298-74G
APE (Ref-1, Apurinic Apyrimidinic Endonuclease, APEX, APE-1, HAP1)
Description:
Applications:
Suitable for use in Immunohistochemistry (parafffin), Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:100.
Western Blot: 1:1000. Detects a band of ~37kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a6D123Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant human APE1 (apurinic/apyrimidinic endonuclease).
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes human APE1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Elevated and altered expression of the multifunctional DNA base excision repair and redox enzyme Ape1/ref-1 in prostate cancer: M.R. Kelley, et al.; Clin. Cancer Res. 7, 824 (2001)
2. Alterations in the expression of the DNA repair/redox enzyme APE/ref-1 in epithelial ovarian cancers: D.H. Moore, et al.; Clin. Cancer Res. 6, 602 (2000)