Technical Data
A2298-74L
APE (APEX, APEX1, APEX nuclease multifunctional DNA repair enzyme, APX, APE1, APEN, HAP1, REF1, REF-1, AP lyase, AP endonuclease class I, DNA apurinic/apyrimidinic exonuclease/ lyase)
Description:
Reactive oxygen species (H2O2, OH, O2, HOCL) generated during normal cellular activities or by ionizing radiation and by many oxidizing/alkylating agents can induce a wide variety of lesions in DNA ranging from oxidized bases (8-oxoguanine and thymine glycol), base loss and DNA strand breaks with modified ends such as 3-phosphate and 3-phopshoglycolate. Unrepaired damaged/modified DNA has been implicated in aging and cancer. Many proteins that are involved in BER (base excision repair) have been identified in E coli and mammals. Repair of modified is initiated by their removal by specific DNA glycosylase resulting in AP (apurinic/apyrimidinic) sites. A series of steps starting with the cleavage of the DNA strand adjacent to AP sites, removal of the deoxyribose phosphate residue, followed by gap filling synthesis and ligation. The DNA strand cleavage is catalyzed by a variety of AP-endonucleases (APE or HAP1, APEX, APE-1) that cleave 5 to the AP sites. APE also has an associated 3-endonuclease activity the removes 3trans-a,b-unsaturated aldehyde generated by AP lyase, as well as 3-phosphate and 3-phosphoglycolate. ROS generated 3-blocked termini at the site of strand breaks are also removed APE.

Applications:
Western Blot: An initial dilution of 1:1K-2K is recommended for Western. The antibody has detected ~34kD band from human cells and tissues.
ELISA: 1:10-50K; using 50-100ng control peptide/well.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Full length human APE expressed in E. coli (36kD)
Purity:
Serum
Form
Supplied as a lyophilized powder.
Specificity:
Recognizes human APE. Reacts poorly with mouse and rat APE.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Seki S et al (1992) BBA 1131, 287-299; Akiyama K et al (1994) BBA 1219, 15-25; Izumi T et al (1996) Biochem. 35, 14679-14683; Wilson DM & Thompson LH (1997) PNAS 94, 12754-1275