		Technical Data
A2298-74Q	APE (APEX, APEX1, APEX Nuclease Multifunctional DNA Repair Enzyme, APX, APE1, APEN, HAP1, REF1, REF-1, AP Lyase, AP Endonuclease Class I, DNA Apurinic/Apyrimidinic Exonuclease/Lyase)

Description:
Apurinic / Apyrimidinic Endonuclease (APE 1 or REF 1) is responsible for the incision of DNA at abasicsites during base excision repair. It has also been shown to stimulate the sequence-specific DNA binding activity of Fos-Jun (AP 1) heterodimers. Alterations in APE 1 levels have been described insome cancers.

Positive Control: HeLa, HepG2 and Raji whole cell lysate.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000-1:10000.
Western Blot: 1:1000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG	8J283	Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Recombinant protein encoding full length human APE 1 purified from E. coli.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2.

Specificity:

This antibody is specific for APE1. Species Crossreactivity: Crossreacts with Human.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Wilson DM & Barsky D The major human abasic endonuclease: formation, consequences and repair of abasic lesions in DNA. Mutat Res 485:283-307 (2001).
Xu Y et al. The apurinic/apyrimidinic endonuclease (APE/ref-1) DNA repair enzyme is elevated in premalignant and malignant cervical cancer. Anticancer Res 17:3713-19 (1997).