		Technical Data
A2298-74Q2	APE (APEX, APEX1, APEX Nuclease Multifunctional DNA Repair Enzyme, APX, APE1, APEN, HAP1, REF1, REF-1, AP Lyase, AP Endonuclease Class I, DNA Apurinic/Apyrimidinic Exonuclease/Lyase)

Description:
Cellular Localization: Nuclear

Positive Control: HeLa and Raji total cell lysates.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Recombinant human APE 1 protein purified from E.coli.

Purity:

Whole antisera

Form

n/a

Specificity:

Species Crossreactivity: Crossreacts with Human.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Wilson DM & Barsky D The major human abasic endonuclease: formation, consequences and repair of abasic lesions in DNA. Mutat Res 485:283-307 (2001). Xu Y et al. The apurinic/apyrimidinic endonuclease (APE/ref-1) DNA repair enzyme is elevated in premalignant and malignant cervical cancer. Anticancer Res 17:3713-19 (1997).