Technical Data
A2298-74W
Atg3 (Autophagy-related Protein 3, APG3, ATG3 Autophagy Related 3 Homolog (S. cerevisiae), APG3-like, AUT1, Protein PC3-96)
Description:
GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. Belongs to the ATG3 family.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:50-1:3000
Immunofluorescence: 1:50-1:3000
Western Blot: 1:50-1:3000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to the central region of human ATG3, conjugated to Blue Carrier Protein. Cellular Localization: Cytoplasm. Species Sequence Homology: mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (canine).
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute with 100ul of sterile ddH2O.
Specificity:
Recognizes human ATG3 at ~37kD. Species Crossreactivity: rat and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tanida I, et al. J. Biol. Chem. 277:13739-13744(2002). 2. Ota T, et al. Nat. Genet. 36:40-45(2004). 3. Rush J, et al. Nat. Biotechnol. 23:94-101(2005).