Technical Data
A2298-75A
Apg12 (Autophagy-related 12, 4931423H11Rik, A330058M13Rik, Atg12, Apg12L, APG12-like, Atg12L, Autophagy-related protein 12)
Description:
Autophagy is the major pathway for proteins and organelles being delivered to the lysosome or vacuole for degradation. Autophagy-mediated degradation may be important during mammalian embryogenesis, differentiation, aging, and non-apoptotic programmed cell death. Dysfunction in autophagic degradation has been observed in a number of genetic diseases, particularly in cancer, and may also be associated with neurodegenerative conditions such as Parkinson’s and Alzheimer’s disease. The Apg12 system is a ubiquitin-like protein conjugation system, first discovered in autophagy-defective yeast mutants, that is conserved in eukaryotes. Covalent attachment of Apg12-Apg5 is required for autophagy; the Apg7 and Apg10 enzymes that catalyze this attachment are analogous to the E1 and E2 enzymes of the ubiquitin-proteasome degradation pathway.

Applications:
Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested.

Recommended Dilutions:
ELISA: 0.1-1ug/ml
Immunoprecipitation (Native): 7ug/reaction
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide derived from the internal region of the human Apg12 protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human Apg12 protein at ~22kD, as well as additional bands between 40 and 80kD of unknown origin. Reactivity has been confirmed with Apg12-transfected, overexpressed HEK293 cell lysates. Species Sequence Homology: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kim, J., et al., J. Biol. Chem. 277(1): 763-773 (2002). 2. Liang, X.H., et al., Nature 402: 672-676 (1999). 3. Cataldo, A.M., et al., Neuron 14: 671-680 (1995). 4. Anglade, P., et al., Histol. Histopathol. 12: 25-31 (1997). 5. Mizushima, N., et al., Int. J. Biochem. Cell Biol. 35(5): 553-561 (2003). 6. Mizushima, N., et al., J. BIol. Chem. 273(51): 33,889-33,892 (1998).