Technical Data
Atg12 (Autophagy-related protein 12, Atg12, Apg12L, APG12-like)
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves an ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin-E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween-20 at 4C with gentle shaking, overnight.
Immunoprecipitation: 1:100.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to residues surrounding Ser36 of human Atg12 protein (KLH- coupled).
Supplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.
Recognizes human endogenous levels of total free Atg 12 and Atg12 present in Atg5-Atg12 complex. Species crossreactivity: mouse, rat, monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21, 2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ12 Suppl 2, 1509-18, 3. Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88, 4. Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92, 5. Mizushima, N. et al. (1998) Nature 395, 395-8, 6. Suzuki, K. et al. (2001) EMBO J 20, 5971-81, 7. Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79, 8. Shintani, T. et al. (1999) EMBO J 18, 5234-41.