Technical Data
A2298-75B
Apg12 (Autophagy-related 12, 4931423H11Rik, A330058M13Rik, Atg12, Apg12L, APG12-like, Atg12L, Autophagy-related protein 12)
Description:
Required for autophagy. SUBUNIT: Conjugated to ATG5.

Cellular Localization:
Cytoplasm.

Alternative Products:
2 named isoforms produced by alternative splicing.

Tissue Specificity:
Ubiquitous. Belongs to the ATG12 family.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide corresponding to the N-terminal of human APG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
Specificity:
IHC and WB confirmed the specificity for APG12. Species Crossreactivity: Human, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mizushima, N. et al. J. Biol. Chem. 273(51):33889-33892 (1998). ; 2. Tanida, I. et al. Biochem. Biophys. Res. Commun. 296(5):1164-1170 (2002). ; 3. Tanida, I. et al. J. Biol. Chem. 277(16):13739-13744 (2002).