		Technical Data
A2298-75D	Apg10 (Autophagy-related Protein 10, Autophagy 10-like, APG10-like, Atg10)

Description:
APG10 plays a role in autophagy. ATG12-conjugating enzyme (E2-like enzyme), likely serves as an ATG5-recognition molecule. SUBUNIT: Interacts with MAP1LC3A. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. Also able to directly interact either with ATG5 or ATG7.

Applications:
Suitable for use in ELISA, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immuhistochemistry (IF): 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Cellular Localization:
Cytoplasm (Probable).

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to the C-terminal of human APG10 protein

Purity:

Serum

Form

Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.

Specificity:

Recognizes human APG10. Species Crossreactivity: human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Wan D. et al. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729 (2004) ; 2. Ota T. et al. Nat. Genet. 36:40-45(2004) ; 3. The MGC Project Team. Genome Res. 14:2121-2127(2004).