Technical Data
A2298-75D
Apg10 (Autophagy-related Protein 10, Autophagy 10-like, APG10-like, Atg10)
Description:
APG10 plays a role in autophagy. ATG12-conjugating enzyme (E2-like enzyme), likely serves as an ATG5-recognition molecule. SUBUNIT: Interacts with MAP1LC3A. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. Also able to directly interact either with ATG5 or ATG7.

Applications:
Suitable for use in ELISA, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immuhistochemistry (IF): 1:100-1:1000
Optimal dilutions to be determined by the researcher.


Cellular Localization:
Cytoplasm (Probable).

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to the C-terminal of human APG10 protein
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
Specificity:
Recognizes human APG10. Species Crossreactivity: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wan D. et al. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729 (2004) ; 2. Ota T. et al. Nat. Genet. 36:40-45(2004) ; 3. The MGC Project Team. Genome Res. 14:2121-2127(2004).