Technical Data
A2298-75D1
Apg10 (Autophagy-related Protein 10, Autophagy 10-like, APG10-like, Atg10)
Description:
ATG10 is an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugation and the modification of a soluble form of MAP-LC3, a homolog of yeast Apg8, to a membrane-bound form. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1903-30U: IgG, H&L, X-adsorbed (HRP) ShxRb

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to a 15 aa sequence from near the carboxy terminus of human ATG10.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human Autophagy-related protein 10 (ATG10). Detects a band of approximately 33 kDa in SK-N-SH cell lysate. Species Crossreactivity: mouse, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.