Technical Data
A2298-75G
APG10L (Ubiquitin-like-conjugating Enzyme ATG10, Autophagy-related Protein 10, APG10-like, ATG10, PP12616)
Description:
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes/vacuoles. The formation of autophagosomes involves a dynamic rearrangement of the membrane for which two ubiquitin-like modifications (the conjugation of APG12p and the modification of a soluble form of MAP-LC3 to a membrane-bound form) are essential. APG10L is an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation, ATG12-ATG5 conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A), a homolog of yeast Apg8, to a membrane-bound form. The protein is ubiquitously expressed. Human APG10L gene is localized on chromosome 5q14.2.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 2-6ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Brain

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa1-50 of human APG10L.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human APG10L. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nemoto, T. et al. J. Biol. Chem. 278:39517-39526 (2003). 2. Baehrecke, EH. et al. Nat. Rev. Mol. Cell. Biol. 6:505-510 (2005).