Technical Data
A2298-76
APG5 (APG5 Autophagy 5-like, APG5-like, APG5L, Apoptosis-specific Protein, ASP, ATG5 Autophagy-related 5 Homolog, Autophagy Protein 5, hAPG5, Homolog of S Cerevisiae Autophagy 5)
Description:
Autophagy is an important mechanism for nonselective intracellular breakdown whereby cytosol and organelles are encapsulated in vesicles, which are then engulfed and digested by lytic vacuoles/lysosomes. ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:500
Immunohistochemistry: 2.5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control: Mouse wildtype ES cell lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide made to an N-terminal region (within residues 1-50) of the human ATG5 protein. (Swiss-Prot# Q9H1Y0)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% sodium azide, 30% glycerol.
Specificity:
Recognizes ATG5. Selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing aa1-79. The calpain cleaved form of ATG5 is missing aa195-275. Species sequence homology: human, rat, bovine, porcine, monkey and Xenopus, 100%; zebrafish, 94%
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mizushima, N., et al. Dissection of autophagosome formation using Apg5- deficient mouse embryonic stem cells. J. Cell. Biol. 152: 657-668 (2001)