Technical Data
A2298-76A
APG5 (APG5 Autophagy 5-like, APG5-like, APG5L, Apoptosis-specific Protein, ASP, ATG5 Autophagy-related 5 Homolog, Autophagy Protein 5, hAPG5, Homolog of S Cerevisiae Autophagy 5)
Description:
Autophagy is a catabolic process for the autophagosomic- lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin-E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to residues near the carboxyl-terminus of Atg5. (KLH)
Purity:
Purified by peptide affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity:
Recognizes endogenous levels of total human Atg5. The observed band represents the Atg12-Atg5 conjugated form, but likely reacts with free Atg5 as well. Species sequence homology: monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1,11–21.(2) Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509–18.(3) Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679–88.(4) Mizushima, N. et al. (1998) J Biol Chem 273, 33889–92.(5) Mizushima, N. et al. (1998) Nature 395, 395–8.(6) Suzuki, K. et al. (2001) EMBO J 20, 5971–81.(7) Tanida, I. et al. (1999) Mol Biol Cell 10, 1367–79.(8) Shintani, T. et al. (1999) EMBO J 18, 5234–41.