		Technical Data
A2298-76B	Apg12 (Autophagy-related 12, 4931423H11Rik, A330058M13Rik, Atg12, Apg12L, APG12-like, Atg12L, Autophagy-related protein 12)

Description:
Autophagy is a catabolic process for the autophagosomic- lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 (APG12) is covalently bound to Atg5 (APG5-like) and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin-E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Immunofluorescence (IF-IC): 1:100
Western Blot: 1:1000
Immunoprecipitation: 1:25
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Mouse	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to residues near the amino terminus of mouse Apg12 (KLH).

Purity:

Purified by peptide affinity chromatography.

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Specificity:

Recognizes endogenous levels of total free and Apg5-like bound mouse Apg12.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21. 2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18. 3. Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88. 4. Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92. 5. Mizushima, N. et al. (1998) Nature 395, 395-8. 6. Suzuki, K. et al. (2001) EMBO J 20, 5971-81. 7. Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79. 8. Shintani, T. et al. (1999) EMBO J 18, 5234-41.