Technical Data
A2298-76B
Apg12 (Autophagy-related 12, 4931423H11Rik, A330058M13Rik, Atg12, Apg12L, APG12-like, Atg12L, Autophagy-related protein 12)
Description:
Autophagy is a catabolic process for the autophagosomic- lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 (APG12) is covalently bound to Atg5 (APG5-like) and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin-E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Immunofluorescence (IF-IC): 1:100
Western Blot: 1:1000
Immunoprecipitation: 1:25
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceMouseRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues near the amino terminus of mouse Apg12 (KLH).
Purity:
Purified by peptide affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity:
Recognizes endogenous levels of total free and Apg5-like bound mouse Apg12.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21. 2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18. 3. Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88. 4. Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92. 5. Mizushima, N. et al. (1998) Nature 395, 395-8. 6. Suzuki, K. et al. (2001) EMBO J 20, 5971-81. 7. Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79. 8. Shintani, T. et al. (1999) EMBO J 18, 5234-41.