Technical Data
A2298-76D
Apg12 (Autophagy-related 12, 4931423H11Rik, A330058M13Rik, Atg12, Apg12L, APG12-like, Atg12L, Autophagy-related protein 12)
Description:
Autophagy is a catabolic process for the autophagosomic- lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Apg12 is covalently bound to Apg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin-E1-like enzyme Apg7 and the E2-like enzyme Apg10 (7,8).

Applications:
Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:100
Western Blot: 1:1000
Immunoprecipitation: 1:25
Optimal dilutions to be determined by the researcher.

Storage and Stability:
For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues near the amino terminus of human Atg12 (KLH).
Purity:
Purified immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity:
Detects endogenous levels of total free at 16kD and Atg5 bound Atg12 protein at 53kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1,1121. 2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ12 Suppl 2, 150918. 3. Levine, B. and Yuan, J. (2005) J Clin Invest 115,267988. 4. Mizushima, N. et al. (1998) J Biol Chem 273,3388992. 5. Mizushima, N. et al. (1998) Nature 395, 3958. 6. Suzuki, K. et al. (2001) EMBO J 20, 597181. 7. Tanida, I. et al. (1999) Mol Biol Cell 10, 136779. 8. Shintani, T. et al. (1999) EMBO J 18, 523441.