Technical Data
A2298-76D1
APG12L (ATG12, Ubiquitin-like Protein ATG12, Autophagy-related Protein 12, APG12-like, APG12)
Description:
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. APG12L is the human homologue of yeast APG12, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy. The protein possesses 27% identity and 48% similarity with the yeast APG12p. Northern blot analysis confirms the ubiquitous expression of the protein in human tissues. The gene encoding human APG12L protein is localized on chromosome 5q21-q22.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to aa84-101 (SAAVSPGTEEPAGDTKKK) of human APG12L.
Purity:
Serum
Form
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Specificity:
Recognizes human APG12L. Species Crossreactivity: chimpanzee, canine. Species sequence homology: chicken, mouse, rat, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Noboru, M. et al. J. Biol. Chem. 273:33889-33892 (1998).