		Technical Data
A2298-76F	APG5 (APG5 Autophagy 5-like, APG5-like, APG5L, Apoptosis-specific Protein, ASP, ATG5 Autophagy-related 5 Homolog, Autophagy Protein 5, hAPG5, Homolog of S Cerevisiae Autophagy 5)

Description:
Autophagy is an important mechanism for nonselective intracellular breakdown whereby cytosol and organelles are encapsulated in vesicles, which are then engulfed and digested by lytic vacuoles/lysosomes. ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

A synthetic peptide corresponding to the C-terminal of human APG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.

Purity:

Serum

Form

Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.

Specificity:

IHC and WB confirmed the specificity for APG5. Species Crossreactivity: Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Mizushima, N et al. (2003) Int J Biochem Cell Biol. 35(5), 553-61 ; 2. Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) ; 3. Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) ; 4. Greenberg JT. Dev Cell. 8(6):799-801. (2005)