		Technical Data
A2298-76H	APG5 (APG5 Autophagy 5-like, APG5-like, APG5L, Apoptosis-specific Protein, ASP, ATG5 Autophagy-related 5 Homolog, Autophagy Protein 5, hAPG5, Homolog of S Cerevisiae Autophagy 5) (HRP)

Description:
Autophagy is an important mechanism for nonselective intracellular breakdown whereby cytosol and organelles are encapsulated in vesicles, which are then engulfed and digested by lytic vacuoles/lysosomes. ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500
Immunohistochemistry: 2.5ug/ml
Optimal dilutions to be determined by researcher.

Positive Control:
Mouse wild-type ES cell lysate.

Storage and Stability:
Store at 2-8°C. Do Not Freeze! Aliquots are stable for at least 3 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		4°C Do Not Freeze	Blue Ice	Human	Rabbit

Concentration:

1.17mg/ml

Immunogen:

A synthetic peptide made to an N-terminal region (aa1-50) of the human ATG5 protein.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, 30% glycerol. Labeled with HRP.

Specificity:

Selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing aa1-79. The calpain cleaved form of ATG5 is missing aa195-275. Cellular Localization: Cytoplasm. Co-localizes with non-muscle actin. Species Crossreactivity: human, mouse, rat, bovine, porcine, primate, Xenopus and Zebrafish. MW of human antigen: 33kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Wang, Y., R. Singh, et al. (2008) J. Biol. Chem. 283:4766-4777. 2. Pyo, J., Nah, J., Kim, H., et al. (2008) Autophagy. April 4(3):315-321. 3. Cadwell K, Liu JY, Brown SL, et al. (2008) Nature 456(7219):259-263. 4. Mizushima, N., et al. (2001) J. Cell. Biol. 152:657-668.