Technical Data
Atg16L1 (Autophagy-related Protein 16-1, ATG16 Autophagy Related 16-like 1, APG16-like 1, APG16 Autophagy 16-like 1)
APG16 L1 plays an essential role in autophagy. SUBUNIT: Homooligomer. Interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400kD) or 8 sets of ATG12-ATG5 and ATG16L1 (800kD).

Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:100-1:3000
Immunohistochemistry: 1:100-1:3000
Western Blot: 1:100-1:3000
Optimal dilutions to be determined by the researcher.

Cellular Localization:
Cytoplasm. Preautophagosomal structure; preautophagosomal structure membrane; peripheral membrane protein. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanRabbit
Synthetic peptide corresponding to the N-terminal of human ATG16-L1 protein conjugated to Blue Carrier Protein.
Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
Recognizes human ATG16 L1. Peptide Sequence Homology: mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zheng H, et al. DNA Seq. 15:303-305(2004). ; 2. Clark H.F, et al. Genome Res. 13:2265-2270(2003). ; 3. Ota T, et al. Nat. Genet. 36:40-45(2004).