Technical Data
APG16L (Autophagy-related Protein 16-1, APG16-like 1, ATG16L1, UNQ9393/PRO34307)
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes/vacuoles. The process is found in eukaryotes, including yeast, and involves several genes and two novel ubiquitin-like conjugation systems. The APG12 system is one of the ubiquitin-like protein conjugation systems essential for autophagy. Covalent attachment of APG12-APG5 is essential for autophagy. In mammalian cells, the APG12-APG5 conjugate is a protein complex of approximately 800kD and includes an additional novel protein. This novel protein was designated APG16L (APG16-like, also referred to as ATG16L) as it's N-terminal region contains several features similar to yeast APG16. APG16L protein has several spliced isoforms and is larger than yeast APG16 (17kD). The protein has a large C-terminal domain containing seven WD repeats. Genetic variations in the protein are associated with susceptibility to inflammatory bowel disease type 10. The human APG16L gene is localized in the chromosomal region 2q37.1.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human skeletal muscle lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to aa25-75 of human APG16L.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Recognizes human APG16L. Species Crossreactivity: chimpanzee, monkey, mouse. Species sequence homology: chicken and canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mizushima, N. et al. Nature. 395:395-398 (1998). 2. Mizushima, N. et al. The International Journal of Biochemistry & Cell Biology. 35:553-561 (2003).