Technical Data
A2298-81C
APOA5 (Apolipoprotein A5, Apo A-V, Apolipoprotein A-V, ApoA-V)
Description:
Apolipoprotein A5 (ApoA5) is fast gaining attention as a key regulator of serum triglyceride concentrations. An ApoA5 mouse knock-out model produced an approximately four-fold increase in serum triglycerides, whereas a knock-in model with human ApoA5 produced 50-70% lower concentrations of mouse serum triglycerides. In addition, peroxisome proliferator-activated receptor-4 (PPAR-alpha) agonists, which are used clinically to lower serum triglyceride concentrations, cause increased ApoA5 mRNA expression. Recently, it was demonstrated that ApoA5 is present in human serum detected by polyclonal antibodies against both the NH2 and COOH termini, although at much lower concentrations than other apolipoproteins.

Positive Control: HEPG2 cells

Applications:
Suitable for use in ECL, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: a band at ~ 41kD is seen.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k8J288Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~4mg/ml
Immunogen:
Ni-NTA purified full-length recombinant human APOA5 expressed in E. coli strain BL21 (DE3)
Purity:
Mouse ascites
Form
As reported
Specificity:
Species Crossreactivity: Human. Other species have not been tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Prieur, X., et al. J. Biol. Chem. 278: 25468-25480 (2003).
2. Obrien, P.J., et al. Clin. Chem. 51: 351-359 (2005).