Technical Data
A2299-08U
Apolipoprotein A-I (Apo A-I)
Description:
Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.

Applications:
Suitable for use in RID and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
RID: 8ul/cm
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanSheep
Concentration:
Not Determined
Immunogen:
Human Apolipoprotein A-1 purified from plasma and shown to be homogeneous by SDS-PAGE. Cellular Localization: Secreted
Purity:
Purified
Form
Supplied as a liquid in GBS, pH 7.4, 0.1% EACA, 0.01% Benzamidine, 1mM EDTA, 0.09% sodium azide.
Specificity:
Recognizes human Apolipoprotein A-1. A single arc is produced when tested by IEP and 2D-IEP against human plasma. Identity is confirmed by double diffusion (Ouchterlony) vs human plasma and a known anti-human Apolipoprotein A-1. Species Crossreactivity: rabbit and canine. Does not crossreact with rat, equine, mouse, goat, bovine, porcine, feline, guinea pig or chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.