Technical Data
A2299-11E
APOL1 (BH3 Domain) (Apolipoprotein L1, Apolipoprotein L-I, ApoL-I, Apolipoprotein L, Apo-L, ApoL)
Description:
APOL1 is a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Flow Cytometry: 1:10-1:50
Immunohistochemistry: 1:50-1:100
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to a portion of amino acids within aa147-176 from the BH3 Domain region of human APOL1, conjugated to KLH (O14791).
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human APOL1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Li,Q., Clin. Chim. Acta 403(1-2), 152-155 (2009). 2. Zhaorigetu,S., Autophagy 4(8), 1079-1082 (2008).