Technical Data
Apolipoprotein B (Apo B)
Lipids (triglyceride (TG), cholesterol (Chol), phospholipids (PL), cholesteryl esters (CE)) are insoluble in plasma. These lipids combine with apolipoproteins to form lipoproteins. This process solubilizes lipids in plasma. Lipoproteins provide a transport system for lipids. They also play an important role in lipoprotein receptor recognition and regulation of certain enzymes in lipoprotein metabolism. Apolipoprotein B plays an essential role in lipid transport and metabolism. Apo B may regulate cholesterol synthesis through its interaction with specific cell membrane receptors and by inhibition of HMG Co A reductase. This enzyme has been identified as the rate controlling enzyme in cholesterol biosynthesis. Apo B may be important in the genesis of atherosclerosis.

Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000-1:10000
Western Blot: 1:200-1:1000
Immunoprecipitation: 1:100
Immunohistochemistry (Paraffin embedded tissue): 1:50-1:500
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-28K: IgG, H&L (HRP) Pab RbxGt
I1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab RbxGt

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Apolipoprotein Type B isolated from human plasma by density gradient centrifugation followed by HPLC puri cation.
Purified by immunoaffinity chromatography.
Supplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 0.01% sodium azide. No stabilizing proteins added.
Recognizes human apolipoprotein B. Assay by IEP resulted in a single precipitin arc against anti goat serum. Has negligible crossreactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apolipoproteins. Typically less than 1% crossreactivity against other types of apolipoprotein was detected by ELISA against puri ed standards. Non-speci c crossreaction of anti-apolipoprotein antibodies with other human serum proteins is negligible.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.