Technical Data
A2299-52M
APOB (Apolipoprotein B-100, Apo B-100)
Description:
Apolipoprotein B (ApoB) is the main apolipoprotein of chylomicrons and low density lipoproteins (LDL). The protein occurs in the plasma in 2 main isoforms, apoB-48 and apoB-100. The first is synthesized exclusively by the gut, the second by the liver. The intestinal (B-48) and hepatic (B-100) forms of apoB are coded by a single gene and by a single mRNA transcript larger than 16kb. The 2 proteins share a common amino terminal sequence. In the ApoB-100 isoform the precursor has 4563aa, and the mature apoB-100 has 4536aa residues. Mature, circulating B-48 is homologous over its entire length (estimated to be between 2130 and 2144aa residues) with the amino-terminal portion of B-100 and contains no sequence from the carboxyl end of B-100. From structural studies, it is thought that apoB-48 represents the amino-terminal 47% of apoB-100 and that the carboxyl terminus of apoB-48 is in the vicinity of residue 2151 of apoB-100. Apolipoprotein B-48 may be the product of an intestinal mRNA with an in-frame UAA stop codon resulting from a C-to-U change in the codon CAA encoding Gln(2153) in apoB-100 mRNA. Since only the sequence that codes B-100 is present in genomic DNA, this presents the possibility of an organ-specific introduction of a stop codon to an mRNA and the change from CAA to UAA of codon 2153 of the message as a unique RNA editing process.

Applications:
Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000-1:10,000
Western Blot: 1:2000-1:10,000
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanGoat
Concentration:
As reported
Immunogen:
APOB isolated from human plasma by density gradient centrifugation followed by HPLC purification.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 0.01% sodium azide.
Specificity:
Recognizes human APOB. Negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J Apolipoproteins and other human serum proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.