Technical Data
A2299-75E1
APOE (Apolipoprotein E, APO-E)
Description:
ApoE is a 35-40kD protein component of serum chylomicrons, VLDL, and HDL particles. It mediates the binding, uptake, and catabolism of these particles through interactions with the ApoE receptor and LDL receptors in the liver and brain. ApoE is important in fatty acid homeostasis and memory formation. Polymorphisms encode three variants (ApoE2, 3, 4) which are differentially related to the development of atherosclerosis and neurogenerative disorders. Mature human ApoE shares 71% aa sequence identity with mouse and rat ApoE.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000-1:40,000
Western Blot: 1:5000-1:40,000
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabPurified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanGoat
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding to human APOE.
Purity:
Purified
Form
Supplied as a liquid in 75mM PBS, 75mM sodium chloride, 0.02% sodium azide, pH 7.2.
Specificity:
Recognizes human APOE.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.