		Technical Data
A2299-76D	Apolipoprotein E,E2,E3,E4 (Apo E,E2,E3,E4)

Description:
Applications:
Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:1000-1:10,000
Immunohistochemistry (Frozen and formalin-fixed paraffin-embedded tissues): 1:1000-1:10,000
The staining intensity of formalin-fixed paraffin embedded tissues may be significantly improved by the pretreatment of:
1. Formic acid (70%) for 10-30 minutes at RT.
2. HIER (Heat Induced Epitope Retrieval) with buffers.
Optimal dilutions to be determined by the researcher.

Source:
Tissue culture supernatant

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	2B16	Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide spanning the polymorphic aa position 158 of Apo E.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added.

Specificity:

Recognizes human Apo E. Recognizes E2, E3 and E4 isoforms of apolipoprotein E. Does not react with mouse and rabbit.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

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