Technical Data
A2299-90K
Apoptosis-Associated Tyrosine Kinase (AATK)
Description:
Apoptosis-associated tyrosine kinase (AATK) is a 155kD membrane-associated intracellular protein that promotes neurite extension and neuronal differentiation. Low extracellular potassium levels induce AATK phosphorylation and apoptosis in cerebellar granule cells. AATK negatively regulates the Na-K-2Cl cotransporter by promoting its PP1-dependent dephosphorylation. The two alternate splice forms of AATK also contain the sequences included in this immunogen. Within this region, human AATK shares 63% aa sequence identity with mouse AATK.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 25ug/ml use 10ul to label 10e6 cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b8H200Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
E. coli-derived rhAATK. (aa 1039 - 1207)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
Specificity:
Recognizes human AATK.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.