Technical Data
A2299-98A
APRIL, Recombinant, Mouse (a Proliferation Inducing Ligand, TALL-2, TNF and ApoL-related Leukocyte-expressed Ligand 2, TRDL-1a, TNF-related Death Ligand 1a, TNFSF13)
5ug
20ug
Molecular Biology Storage: -20CShipping: RT
APRIL (a proliferation-inducing Ligand), also known as TNFSF13, is a type II membrane protein belonging to the TNF Ligand family. Although low levels of APRIL are found in normal tissues, it is abundantly expressed in tumor cells. Recombinant soluble APRIL stimulates proliferation in Raji B cells, Jurkat T cells and MCF-7 carcinoma cells. Both APRIL and BAFF (BLyS) mediate their distinct biological roles through two receptors, TACI and BCMA. Recombinant Murine APRIL is a soluble 21.9kD protein, consisting of 192 amino acid residues.

Biological Activity:
Measured by its ability to induce cell proliferation of activated T cells.

AA Sequence:
MRREVSRLQR SGGPSQKQGE RPWQSLWEQS PDVLEAWKDG AKSRRRRAVL TQKHKKKHSV LHLVPVNITS KDSDVTEVMW QPVLRRGRGL EAQGDIVRVW DTGIYLLYSQ VLFHDVTFTM GQVVSREGQG RRETLFRCIR SMPSDPDRAY NSCYSAGVFH LHQGDIITVK IPRANAKLSL SPHGTFLGFV KL

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with ddH2O, 0.1% BSA or HSA. Aliquot and store at -20C. Reconstituted product is stable for 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
21.9kD
Source: Recombinant, Murine expressed in E. coli
Purity: 98% by SDS-PAGE and HPLC analyses Endotoxin: 0.1ng/ug (1EU/ug)
Form: Supplied as a lyophilized powder from 10mM sodium phosphate, pH 5, 100mM arginine. Reconstitute in sterile dH2O to a concentration of 0.1-1.0mg/ml. This solution can then be diluted into other aqueous buffers with carrier protein. Do not vortex..

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Hahne, M., et al., J. Exp. Med.188(6): 1185-1190 (1998). 2. Shu, H.B., et al., J. Leukoc. Biol. 65(5): 680-683 (1999). 3. Kelly, K., et al., Cancer Res. 60(4): 1021-1027 (2000). 4. Yu, G., et al., Nat. Immunol. 1(3): 252-256 (2000). 5. Wu, Y., et al. J. Biol. Chem. 275(45): 35,478-35,485 (2000). 6. Marsters, S.A., et al., Curr. Biol. 10(13): 785-788 (2000). 7. Rennert, P., et al., J. Exp. Med. 192(11): 1677-1684 (2000). 8. Gross, J.A., et al., Nature 404: 995-999 (2000). 9. Ware, C.F., J. Exp, Med. 192(11): F35-38 (2000).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.