Technical Data
A2300-01A
Aprotinin (Pancreatic trypsin inhibitor)
Description:
Aprotinin, also called bovine pancreatic trypsin inhibitor, is a single chain globular Kunitz-type serine proteinase inhibitor found in bovine pancreas, parotid gland and lung. It forms stable, enzymatically inactive complexes with trypsin, chymotrypsin, plasmin and kallikrein. Aprotinin has been used therapeutically as an antifibrinolytic agent under the name Trasylol (Bayer) to reduce blood loss during cardiopulmonary bypass surgery but this use has been associated with an increased frequency of acute renal injury (1).

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:33,000. 1ug/ml A2300-01A when biotinylated (or A2300-01B), forms an antibody pair with A2300-01C (as capture antibody) for high-sensitivity sandwich ELISA of aprotinin at sample concentrations of 0.6-20ug/ml. Aprotinin in urine diluted 1:1000 can be assayed against standards in buffer. For assay in plasma, it is recommended to add aprotinin-free control plasma to the standards to match the plasma sample dilution.
A2300-01A when biotinylated (or A2300-01B), also forms an antibody pair with A2300-01 (as capture antibody) for sandwich ELISA of aprotinin at samples concentrations of 4-250ug/ml. Both urine and plasma samples diluted 1;1000 can be assayed against standards in buffer. PBS-based buffers are unsuitable for this assay and 0.2M glycine sodium buffer, pH 8.8, is recommended.
Optimal dilutions to be determined by the researcher.

Culture Medium: RPMI 1640 with 2-10% fetal calf serum.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k9A509Affinity Purified
SizeStorageShippingSourceHost
25ul-20CBlue IceBovineMouse
Concentration:
~1mg/ml
Immunogen:
Aprotinin from bovine lung adsorbed onto aluminum hydroxide gel.
Purity:
Purified by Protein A/G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide before the addition of glycerol to 40%.
Specificity:
Binds bovine aprotinin immobilized on a solid phase or free in solution. Epitope is different from A2300-01 and A2300-01C.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mangano DT, Tudor IC, Dietzel C (2006) The risk associated with aprotinin in cardiac surgery. N. Engl. J. Med. 354: 353-365.