		Technical Data
A2300-01B	Aprotinin (Pancreatic trypsin inhibitor) (Biotin)

Description:
Aprotinin, also called bovine pancreatic trypsin inhibitor, is a single chain globular Kunitz-type serine proteinase inhibitor found in bovine pancreas, parotid gland and lung. It forms stable, enzymatically inactive complexes with trypsin, chymotrypsin, plasmin and kallikrein. Aprotinin has been used therapeutically as an antifibrinolytic agent under the name Trasylol (Bayer) to reduce blood loss during cardiopulmonary bypass surgery but this use has been associated with an increased frequency of acute renal injury (1).

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
ELISA: 1:20,000
Optimal dilutions to be determined by the researcher.

Matched Pair:
Capture:
A2300-01C (Samples concentrations: 0.6-20ug/ml)
A2300-01 (Samples concentrations: 4-250ug/ml) PBS-buffers are unsuitable for these assays and 0.2M glycine sodium buffer, pH 8.8, is recommended.
Detection:
A2300-01B

Culture Medium:
RMPI 1640 with 2-10% fetal calf serum

Hybridoma:
Sp2mIL6 myeloma cells with spleen cells from NMRI x Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	9A509	Affinity Purified

Size		Storage	Shipping	Source	Host
25ul		-20°C	Blue Ice	Bovine	Mouse

Concentration:

~1mg/ml

Immunogen:

Aprotinin from bovine lung adsorbed onto aluminum hydroxide gel.

Purity:

Purified by Protein A/G affinity chromatography.

Form

Supplied as a liquid in PBS pH 7.4, 15mM sodium azide. Labeled with Biotin.

Specificity:

Recognizes bovine aprotinin immobilized on a solid phase or free in solution. Epitope is different from A2300-01 and A2300-01C.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Mangano DT, Tudor IC, Dietzel C (2006) The risk associated with aprotinin in cardiac surgery. N. Engl. J. Med. 354: 353-365.