Technical Data
APS is an SH2 and PH domain-containing adaptor protein closely related to Lnk and SH2-B (1). APS was identified as a substrate for many receptor tyrosine kinases including TrkA, insulin receptor, c-Kit and PDGF receptor (2). Tyrosine phosphorylation of APS provides docking sites for downstream signalingcomponents, mediating diverse signaling pathways. APS plays quite different roles in RTK signaling. Overexpression of APS has been shown to inhibit PDGF-induced mitogenicity, which may result from APS/c-Cbl-mediated PDGF receptor degradation (3). However, APS promotes enhanced mitogenicity in response to insulin stimulation (4). The striking difference in APS-mediated signaling between the different RTKs could lie in the mode of interaction with the respective receptor.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide (KLH-coupled) corresponding to residues near the amino-terminus of human APS.
Purified by Protein A and immunoaffinity chromatography.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150 mM NaCl, 100ug/ml BSA and 50% glycerol.
Detects endogenous levels of tota lhuman APS. Does not crossreact with other adaptor/docking proteins. Species sequence homology: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Liu, J. et al. (2002) Mol. Cell. Biol. 22, 35993609. (2) Qian, X. and Ginty, D. (2001) Mol. Cell. Biol. 21, 16131620. (3) Wollberg, P. et al. (2003) Biochem. J. 370, 10331038. (4) Ahmed, Z. and Pillay, T.S. (2001) Biochem. Soc. Trans. 29, 529534.