		Technical Data
A2500-02B	APS, ID (SH2B Adapter Protein 2, SH2 and PH Domain-containing Adapter Protein APS, Adapter Protein With Pleckstrin Homology And Src Homology 2 Domains, SH2B2)

Description:
APS is expressed in B lymphocytes and contains pleckstrin homology and src homology 2 (SH2) domains. In Burkitt lymphoma cell lines, it is tyrosine phosphorylated in response to B cell receptor stimulation. Because it binds Shc independent of stimulation and Grb2 after stimulation, it appears to play a role in signal transduction from the receptor to Shc/Grb2.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
200ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the center region of human APS (KLH).

Purity:

Purified by ammonium sulfate precipitation.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human APS.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Li,M., Endocrinology 148(4), 1615-1621 (2007). 2.Katsanakis,K.D., J. Biol. Chem. 280(45), 37827-37832 (2005). 3.Iseki,M., Biochem. Biophys. Res. Commun. 330(3), 1005-1013 (2005).