Technical Data
A2990
Aquaporin 0 (AQP0, MIP26, Major Intrinsic Protein)
Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporins". Over the last decade, genes for several members of aquaporin family (AQP0, AQP1-15) have been cloned, expressed, and their distribution studied in many tissues. The individual members of aquaporin family have identical predicted secondary structures with up to 6 highly conserved hydrophobic membrane spanning domains (about 18-25aa each) and two conserved NPA motifs. However, N/C-terminal regions of AQPs are only ~20% conserved.

AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 26aa; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents ~80% of total lens membrane protein.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-10ug/ml (ECL)
ELISA: 1:100,000; Use 50-100ng control peptide per well
Optimal dilutions to be determined by researcher

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 17aa within the C-terminal, cytoplasmic domain of human Aquaporin 0, conjugated to KLH. Species Sequence Homology: bovine, 94%; rat and mouse, 88%; frog, 70%.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Specificity:
Recognizes human Aquaporin 0.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pisano, M.M., et al., Genomics 11: 981-990 (1991). 2. Kent, N.A., et al., Nucl. Acid Res. 18: 4256 (1990). 3. Shiels, A., et al. Nucl Acid Res. 16: 9348 (1988). 4. Shiels, A., et al., Nature Genet. 12: 212 (1996).