Technical Data
A2995-16
Aquaporin 0 (AQP0, MIP26)
Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents up to 80% of total lens membrane protein. Defects in MIP26 are cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an amino acid substitution that inhibits targeting of MIP to the cell membrane.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:5000 (ECL)

ELISA: 1:10,000-50,000. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Control Peptide:
A2995-18: Aquaporin 0, Human, Control Peptide (AQP0, MIP26)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgYSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanChicken
Concentration:
As reported
Immunogen:
A synthetic peptide corresponding to 17aa within the carboxy terminal, cytoplasmic domain of human AQP0.
Purity:
Serum
Form
Supplied as a liquid.
Specificity:
The 17 AA peptide was found unique to AQP0 without significant homology to any other AQPs. It has significant homology with bovine (94%), rat/mouse (88%) and frog (70%) AQP0. Antibody crossreactivity in various species is not established. Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Pisano MM et al (1991) Genomics 11, 981-990; Kent NA et al (1990) Nucl. Acid Res. 18, 4256; Shiels A et al (1988) Nucl Acid Res. 16, 9348; Shiels A et al (1996) Nature Genet. 12, 212