		Technical Data
A2999-50B	Aquaporin 1 (AQP1, CHIP-28, Channel-forming Integral Protein)

Description:
Aquaporin 1 is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60kD in mass.

Applications:
Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:1000-1:10,000
Immunoprecipitation: 1:1000-1:10,000
Western Blot: 1:1000-1:2000
Optimal working dilutions to be determined by researcher.

Hybridoma:
Sp2/0-AG14. myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b	3H550	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Rat	Mouse

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to amino acids 198-208 of rat aquaporin 1.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.

Specificity:

Recognizes an epitope within an extracellular region of the water-specific channel aquaporin 1 (also known as AQP1 or CHIP-28). Species Crossreactivity: Human, mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Bodis, B., et al., Journal of Physiology. 95: 271-275 (2001).