Technical Data
A2999-50C
Aquaporin 1 (AQP1, CHIP-28, Channel-forming Integral Protein)
Description:
Aquaporin 1 is a 28kD intergral membrane protein which was originally identified in red blood cells and the kidney. AQP1 is also expressd by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60kD in mass.

Applications:
Suitable for use in Immunohistochemistry, ELISA, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen and paraffin): 1:500-1:1000, Note: Antigen retrieval using heat treatment prior to staining of paraffin section is required. Sodium citrate buffer pH 6.0 is recommended for this purpose.
ELISA: 1:1000-1:20,000
Immunoprecipitation: 1:1000-1:10,000
Western Blot: 1:1000-1:5000
Optimal working dilutions to be determined by researcher.

Recommended Secondary Reagents:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo

Recommended Positive Control:
Kidney

Hybridoma:
Spleen cells from immunized Balb/c mice were fused with the cells of Sp-2/0 Ag14 mouse myeloma cell line.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13H551Affinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa249-269 of rat aquaporin 1.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.09% sodium azide, 0.1% BSA.
Specificity:
Recognizes an epitope within the cytoplasmic domain of the water-specific channel rat aquaporin 1 (also known as AQP1 or CHIP-28). Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bodis, B. et al. (2001). Active water selective channels in the stomach: investigation of aquaporins after ethanol and capsaicin treatment in rats. Journal of Physiology. 95: 271-275. 2. Nagy, G et al., Pathol. Oncol. Res. 8:115-124. (2002).