Technical Data
A3000
Aquaporin 1 (AQP1, CHIP28)
Description:
Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). This gene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayer spanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant in erythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement.

Function: SwissProt: P29972 # Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Size: 269 amino acids; 28526D
Subunit: Homotetramer.
Cellular Localization: Membrane; Multi-pass membrane protein.
Tissue Specificity: Expressed in a number of tissues including erythrocytes, renal.
Entrez Gene Number: NM_198098.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot1-10ug/ml using Chemiluminescence technique. Immunoblots reveal two bands, a lower 28kD band corresponding to unglycosylated form and an upper high molecular 35-60kD glycosylated component (Denker et al. 1988; Smith & Agre 1991) We recommend the use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.
Immunohistochemistry: 2-10ug/ml on paraformaldehyde fixed tissue sections. Rat kidney sections.
ELISA: 0.5-1ug/mL using 1ug/ml control peptide/well.
Optimal dilution determined by the researcher.

Control Antigen:
A3000A: Aquaporin 1 (AQP1, CHIP28), Control Antigen

Molecular Weight: 28kD

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Reconstitution)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A 19 aa synthetic peptide from the cytosolic carboxyl terminal domain of the AQP1 (Lanahan et al. 1992; Deen et al. 1992; Moon et al. 1993; Patil et al. 1994) conjugated to KLH. This peptide contains most of the epitopes recognized by polyclonal antibodie
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.4, 0.025% sodium azide. Reconstitution: Reconstitute with 50ul sterile, distilled, deionized water or sterile 40-50% glycerol for long term storage.
Specificity:
Recognizes Aquaporin 1. Species crossreactivity: Rat and human. Species Sequence Homology: Rat, mouse, bovine, sheep: 100%; Canine: 18/19; Frog (Rana esculenta), toad (Bufo marinus): 14/19.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Quigley, R. et al. (1998) J. Membr. Biol. 164, 177. 2. Mittal, A. et al. (2000) Life Sci., 66, 1471.