Technical Data
A3000-01B
Aquaporin 1 (AQP1, CHIP-28, Channel-forming Integral Protein)
Description:
Aquaporin 1 is a 28kD integral protein thought at first to be a breakdown product of the Rh polypeptide but was later shown to be a unique molecule that is abundant in erythrocytes and renal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000-1:20,000
Western Blot: 1:1000-1:5000; Predicted molecular weight 32.3kD.
Immunoprecipitation: 1:1000
Immunohistochemistry (Frozen): 1:500-1:1000
Immunohistochemistry (Paraffin): 1:500-1:1000. Heat mediated antigen retrieval is required for paraffin sections.
Immunofluorescence (IC): 1:10-1:2000
Optimal dilutions to be determined by the researcher.

Positive Control:
Kidney

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG18J300Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa249-269 of rat Aquaporin 1. Cellular Localization: Plasma membrane
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes an epitope within the cytoplasmic domain of the water-specific channel of rat Aquaporin 1. Species Crossreactivity: human, mouse, rabbit and zebrafish
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Felszeghy, S. et al (2004) Expression of aquaporin isoforms during human and mouse tooth development. 2. Francone, V.P. et al (2010) Signaling from the secretory granule to the nucleus: Uhmk1 and PAM.