		Technical Data
A3000-01B	Aquaporin 1 (AQP1, CHIP-28, Channel-forming Integral Protein)

Description:
Aquaporin 1 is a 28kD integral protein thought at first to be a breakdown product of the Rh polypeptide but was later shown to be a unique molecule that is abundant in erythrocytes and renal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000-1:20,000
Western Blot: 1:1000-1:5000; Predicted molecular weight 32.3kD.
Immunoprecipitation: 1:1000
Immunohistochemistry (Frozen): 1:500-1:1000
Immunohistochemistry (Paraffin): 1:500-1:1000 Heat mediated antigen retrieval is required for paraffin sections.
Optimal dilutions to be determined by the researcher.

Positive Control:
Kidney

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	8J300	Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Rat	Mouse

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide, corresponding to aa249-269 of rat Aquaporin 1. Cellular Localization: Plasma membrane.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid, 1% BSA, 0.09% sodium azide.

Specificity:

Recognizes an epitope within the cytoplasmic domain of the water-specific channel of rat Aquaporin 1. Species Crossreactivity: human, mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Felszeghy, S. et al (2004) Expression of aquaporin isoforms during human and mouse tooth development. 2. Francone, V.P. et al (2010) Signaling from the secretory granule to the nucleus: Uhmk1 and PAM.