Technical Data
Aquaporin 1, Control Peptide (AQP1, CHIP-28, Channel-forming Integral Protein)
Molecular Biology Storage: -20CShipping: Blue Ice
Control peptide for Aquaporin 1, Chicken Anti-rat, serum, polyclonal antibody.

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporins". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents up to 80% of total lens membrane protein.

Synthetic peptide corresponding to 19aa of rat AQP1 (protein accession #P29975)

Molecular Weight:

Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Synthetic peptide
Purity: Purified
Concentration: ~1mg/ml
Form: Supplied as a lyophilized pwder from PBS, pH7.5.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Lanahan, A. et al (1992) MCB, 12:3919- 3929. 2. Deen, P. et al (1992) Biochem.Biophys Res Comm. 188:1267- 1273. 3. Moon, C. et al (1993) JBC. 268:15772-15778. 4. Patil, R. et al (1994) ) Biochem. Biophys Res Comm. 204:861-866. 5. Smith, B. and Agre, P. (1991) JBC 266:6407-6415.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.