Technical Data
A3000-04
Aquaporin 2 (AQP2, AQP-CD, WCH-CD)
Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein “aquaporin.” Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26kD) and Aquaporin-1 (purified from red cells), or CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) have been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. Human Aquaporin-0 is a 263aa transmembrane protein belonging to the MIP family. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N- and C-termini are predicted to be cytoplasmic.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoflourescence. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-10ug/ml (ECL).
ELISA: 0.5-1ug/ml; Coat plates with A3000-07M: Aquaporin 2, Rat, Control Peptide at 1ug/ml
Immunohistochemistry: 2-10ug/ml in paraformaldehyde fixed tissue sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRatRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Synthetic peptide corresponding to15aa within the C-terminus of rat AQP2 conjugated to KLH. Cellular Localization: Cytoplasmic. Species Sequence Homology: mouse; 100%; human and sheep; 93%.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophillized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity:
Recognizes rat Aquaporin 2 (AQP-CD/WCH-CD).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Straube, T. et al., (2011) J. Exp. & Clinical Cancer Research 30:89. 1. Buemi M, 2004, Am Journal of Hypertension 17, 1170-1178, WB, 2. Leung JCK, 2005, Nephrology 10, 63-72, WB,, IHC 3. Ferguson CJ, 2000, Kidney Intl. 64, 1755-1764, WB,, , IF 4. Ross, MJ, 2001, J Am Soc Nephrol 2001 12: 2645-2651, , IHC 5. Saito O, 2003, Am J Physiol Renal Physiol, 284, 1164-1170, , IHC, Schneider A, 2002, Am J Physiol Renal Physiol : 284: 411, IHC 6. van Vonderen IK, 2004, Domestic Animal Endocrinol. 27, 141-153