Technical Data
A3000-04A
Aquaporin 2 (AQP2, AQP-CD, WCH-CD) (Biotin)
Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein “aquaporin”. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26kD) and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) have been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. Human Aquaporin-0 is a 263aa transmembrane protein belonging to the MIP family. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N- and C-termini are predicted to be cytoplasmic.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoflourescence. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml (ECL).
ELISA: 0.5-1ug/ml; Coat plates with A3000-07M Control Peptide at 1ug/ml
Immunohistochemistry: 2-10ug/ml in paraformaldehyde fixed tissue sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceRatRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic 15aa peptide sequence within the C-terminus of rat AQP2. Cellular Localization: Cytoplasmic
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Specificity:
Recognizes rat Aquaporin 2 (AQP-CD/WCH-CD). Species Sequence Homology: Mouse: 100%; Human, Ovine: 93% (14/15aa; residues 257/271 with 1 conservative replacement).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Buemi, M., Am. Journal of Hypertension 17: 1170-1178 (2004) (WB). 2. Leung, J.C.K., Nephrology 10: 63-72 (2005) (WB, IHC). 3. Ferguson, C.J., Kidney Intl. 64: 1755-1764 (2000) (WB, IF). 4. Ross, M.J., J. Am. Soc. Nephrol. 12: 2645-2651 (2001) (IHC). 5. Saito, O., Am. J. Physiol. Renal. Physiol. 284: 1164-1170 (2003) (IHC). 6. Schneider, A., Am. J. Physiol. Renal. Physiol. 284: 411 (2002) (IHC). 7. van Vonderen, I.K., Domestic Animal Endocrinol. 27: 141-153 (2004).