Technical Data
A3000-07A
Aquaporin 2 (AQP2, AQP-CD, WCH-CD) (Biotin)
Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein “aquaporin”. Over the last decade, genes for several members of aquaporin family (AQP1-9 and AQPQP) have been cloned, expressed, and their distribution studied in many tissues.
AQP2 or AQP-CD or WCH-CD (mouse, rat, human 271aa; human gene locus 12q13) is the vasopressin-regulated water channel of the apical membrane of collecting duct cells. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-termini are predicted to be cytoplasmic. Defects in AQP2 are a cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using ECL. AQP2 is ~30kD (3).
ELISA: 0.5-1ug/ml. Conrtol peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
A3000-07D: Aquaporin 2, Mouse, Control Peptide (AQP-CD, WCH-CD)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceMouseRabbit
Concentration:
~0.48mg/ml
Immunogen:
Synthetic peptide corresponding to 19aa at the N-terminal, extracellular domain of mouse AQP2, conjugated to KLH. Species Sequence Homology: rat-; 100%, sheep; 78%, human AQP2; 73%.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative and no stabililizing protein added. Labeled with Biotin.
Specificity:
Recognizes mouse AQP2 at ~30kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fushimi K, et al (1993) Nature 361, 549-552. 2. Nielsen, S et al (1993) PNAS 90, 11663-11667. 3. Deen, PMT et al (1995) J. Clin. Invest. 95, 2291-2296. 4. Sasaki, S et al (1994) J. Clin. Invest. 93, 1250-1256.