Technical Data
A3000-07P
Aquaporin 2 (AQP-CD, WCH-CD)
Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents up to 80% of total lens membrane protein. Defects in MIP26 are cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an amino acid substitution that inhibits targeting of MIP to the cell membrane.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000 using ECL (~30kD)
ELISA: 1:10,000-50,000. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Control Peptide: A3000-07D

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20°CBlue IceMouseRabbit
Concentration:
Not Determined
Immunogen:
A 19 AA peptide sequence mapping at the N-terminal, 2nd extracellular domain of mouse/rat AQP2 conjugated to KLH. Species sequence homology: 78% in sheep and 73% in human AQP2.
Purity:
Serum
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes mouse AQP2 at ~30kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fushimi K, et al (1993) Nature 361, 549-552. 2. Nielsen, S et al (1993) PNAS 90, 11663-11667. 3. Deen, PMT et al (1995) J. Clin. Invest. 95, 2291-2296. 4. Sasaki, S et al (1994) J. Clin. Invest. 93, 1250-1256.