Technical Data
A3000-08
Aquaporin 2, phosphorylated (Ser261) (Aquaporin-2, AQP 2, AQP2, AQP-2, Aquaporin 2 Collecting Duct, ADH Water Channel, Aquaporin-CD, AQP CD, AQP-CD, Collecting Duct Water Channel Protein, MGC34501, Water Channel Protein for Renal Collecting Duct, WCH CD,
Description:
Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C/-20C (No Glycerol)Blue IceRatRabbit
Concentration:
Not determined
Immunogen:
Synthetic phosphopeptide corresponding to aa sequence within Aquaporin 2, which includes phosphorylated Ser261.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM Hepes, pH 7.5, 0.09% sodium azide, 0.01% BSA, 50% glycerol.
Specificity:
Recognizes the 29kD integral membrane rat protein Aquaporin 2 (AQP2), when phosphorylated at Ser261. Species sequence homology: Mouse, Dog, Primate, Human, Bovine, Chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Liu, H. and Wintour, E.M. (2005) Aquaporins in development a review. Reprod. Biol. Endocrinol. 3: 18-27, 2. Hoffert, J.D. et al. (2007) Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am. J. Physiol. Renal Physiol. 292: 691-700.