		Technical Data
A3000-08	Aquaporin 2, phosphorylated (Ser261) (Aquaporin-2, AQP 2, AQP2, AQP-2, Aquaporin 2 Collecting Duct, ADH Water Channel, Aquaporin-CD, AQP CD, AQP-CD, Collecting Duct Water Channel Protein, MGC34501, Water Channel Protein for Renal Collecting Duct, WCH CD,

Description:
Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		4°C/-20°C (No Glycerol)	Blue Ice	Rat	Rabbit

Concentration:

As reported

Immunogen:

Synthetic phosphopeptide corresponding to aa sequence within Aquaporin 2, which includes phosphorylated Ser261.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in 10mM Hepes, pH 7.5, 0.09% sodium azide, 0.01% BSA, 50% glycerol.

Specificity:

Recognizes the 29kD integral membrane rat protein Aquaporin 2 (AQP2), when phosphorylated at Ser261. Species sequence homology: Mouse, Dog, Primate, Human, Bovine, Chicken.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Liu, H. and Wintour, E.M. (2005) Aquaporins in development – a review. Reprod. Biol. Endocrinol. 3: 18-27, 2. Hoffert, J.D. et al. (2007) Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am. J. Physiol. Renal Physiol. 292: 691-700.