Technical Data
A3000-09
Aquaporin 3 (AQP3, GLIP, Glycerol-transporting Integral Protein)
Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein “aquaporin”. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. Human Aquaporin-0 is a 263 amino acid transmembrane protein belonging to the MIP family. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml.
ELISA: 1:1000.
Immunohistochemistry: 2-10ug/ml. See references.
Optimal dilutions to be determined by the researcher.

Control Peptide: A3000-11G

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRatRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Synthetic peptide corresponding to 15aa near the C-terminal, cytoplasmic domainof rat AQP3, conjugated to KLH.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity:
Recognizes rat Aquaporin 3. Species Sequence Homology: mouse; 92%, human; 85%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Proc. Natl. Acad. Sci. (1994) 91:6269–6273 2. Proc. Natl. Acad. Sci. (1994) 91:10997–11000 3. J. Biol. Chem (1994) 269:21845–21849 4. Am. J. Physiol.. (1995) 269: F663-F672. 5. Barcroft LC, 2003, Developemental Biol. 256, 342-354 (IHC). 6. Tamai K, 2006, Res. In Press, (IHC).