Technical Data
A3000-10
Aquaporin 3 (AQP3, GLIP, Glycerol-transporting Integral Protein)
Description:
SwissProt: Q92482. Forms a water-specific channel that provide kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. May function as a water and urea exit mechanism in antidiuresis in collecting duct cells. Also slightly permeable to urea and glycerol. May play an important role in gastrointestinal tract water transport and in glycerol metabolism (By similarity).

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:200 Rat kidney membranes. Identifies a 31kD and 40kD band. The large band may represent the glycosylated form of the protein.
Immunohistochemistry: Mouse kidney sections. Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal dilutions to be determined by the researcher.

Control Antigen: A3000-10A. Included free of charge with the antibody is 20ug of control antigen (lyophilized powder). Reconstitute with 100ul of deionized water. For negative control, preincubate 1ug of purified peptide with 1ug of antibody for one hour at RT.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ul4°C (-20°C Glycerol)Blue IceRatRabbit
Concentration:
~0.3mg/ml
Immunogen:
Synthetic peptide corresponding to aa275-292 [(C)STEA ENVKL AHMKH KEQI] of rat AQP3 (Accession # P47862) Epitope Location: Intracellular, C-terminus.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.4, 1% BSA, 5% sucrose, 0.025% sodium azide. Reconstitution: 50ul sterile dH2O.
Specificity:
Recognizes rat Aquaporin 3 (AQP3, GLIP, Glycerol-transporting Integral Protein). Species Crossreactivity: Mouse. Species Sequence Homology: Mouse: 17/18 residues identical; Sheep, human: 15/18 residues identical.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. PNAS 91: 6269-6273 (1994). 2. PNAS 91: 10997-11000 (1994). 3. J. Biol. Chem. 269: 21845-21849 (1994). 4. Am. J. Physiol. 269: F663-F672 (1995).